Above the chair rail in the room, there is a rare stenciled decoration featuring a vine and berry motif. The windows are framed by thistle leaves, symbolizing from the heart, and vines with pineapples, which symbolize "welcome." The ceiling is bordered by a quilt or Pennsylvania Dutch design. The original Federal-style mantle is still in place and there is a small door opposite the bed, which provides access to the attic of the dining room wing. The original yellow pine wide-plank flooring is still present throughout the second floor.  

The bed in the room is an elaborately carved Rococo-style walnut bed, which was a wedding gift from Dr. Johnston's father-in-law, circa 1855. The coverlet was designed for this bed in 2000 by Jean Bane, who spent over 1000 hours crocheting and sewing together the pinwheel-design cotton squares. Other Johnston family pieces include a 19th-century Renaissance Revival marble-top dresser, a walnut and marble washstand, and an Eastlake walnut wardrobe. The small trunk on the hearth was used by Dr. Johnston's son at college. A walnut folding crib, circa 1875, was a gift to the museum.